Mtp-40 and alpha antigen gene fragment amplification for the detection of Mycobacterium tuberculosis in Colombian clinical specimens
Mem. Inst. Oswaldo Cruz
; 97(8): 1157-1163, Dec. 15, 2002. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-326328
Biblioteca responsável:
BR1.1
RESUMO
In this study, the use of Mtp-40 and alpha antigen polymerase chain reaction (PCR) amplification fragments for the precise tuberculosis (TB) diagnosis was evaluated. One hundred and ninety two different samples were obtained from 113 patients with suspected TB. Mtp-40 and alpha antigen protein genes were amplified by the PCR technique and compared to both the "gold standard" (culture) test, as well as the clinical parameters (including a clinical record and X-ray film exam in 113 patients). Thirty-eight of the 113 patients had a presumptive clinical diagnosis of TB; 74 percent being detected by PCR technique, 58 percent by culture and 44 percent by direct microscopic visualization. Weconclude that it is possible to use PCR as a suitable technique for the detection of any mycobacteria by means of the alpha antigen product, or the specific infection of Mycobacterium tuberculosis by means of the mtp-40 gene. This might be a good supporting tool in difficult clinical TB diagnosis and pauci-bacillary cases
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doenças Negligenciadas
/
Tuberculose
Base de dados:
LILACS
Assunto principal:
Proteínas de Bactérias
/
Tuberculose
/
Mycobacterium tuberculosis
/
Antígenos de Bactérias
Tipo de estudo:
Estudo diagnóstico
/
Estudo de avaliação
Limite:
Humanos
País/Região como assunto:
América do Sul
/
Colômbia
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2002
Tipo de documento:
Artigo
País de afiliação:
Colômbia
Instituição/País de afiliação:
Universidad Nacional de Colombia/CO