Distribution of liposome-encapsulated antimony in dogs
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;36(2): 269-272, Feb. 2003. ilus
Article
em En
| LILACS
| ID: lil-326423
Biblioteca responsável:
BR1.1
ABSTRACT
The achievement of complete cure in dogs with visceral leishmaniasis is currently a great challenge, since dogs are the main reservoir for the transmission of visceral leishmaniasis to humans and they respond poorly to conventional treatment with pentavalent antimonials. In order to improve the efficacy of treatment, we developed a novel formulation for meglumine antimoniate based on the encapsulation of this drug in freeze-dried liposomes (LMA). The aim of the present study was to evaluate the biodistribution of antimony (Sb) in dogs following a single intravenous bolus injection of LMA. Four healthy male mongrel dogs received LMA at 3.8 mg Sb/kg body weight and were sacrificed 3, 48 and 96 h and 7 days later. Antimony was determined in the blood, liver, spleen and bone marrow. In the bone marrow, the highest Sb concentration was observed at 3 h (2.8 æg/g wet weight) whereas in the liver and spleen it was demonstrated at 48 h (43.6 and 102.4 æg/g, respectively). In these organs, Sb concentrations decreased gradually and reached levels of 19.1 æg/g (liver), 28.1 æg/g (spleen) and 0.2 æg/g (bone marrow) after 7 days. Our data suggest that the critical organ for the treatment with LMA could be the bone marrow, since it has low Sb levels and, presumably, high rates of Sb elimination. A multiple dose treatment with LMA seems to be necessary for complete elimination of parasites from bone marrow in dogs with visceral leishmaniasis
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Compostos Organometálicos
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Doenças do Cão
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Leishmaniose Visceral
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Meglumina
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Antiprotozoários
Limite:
Animals
Idioma:
En
Revista:
Braz J Med Biol Res
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Braz. j. med. biol. res
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Braz. j. med. biol. res. (Online)
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Brazilian journal of medical and biological research
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Brazilian journal of medical and biological research (Impresso)
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Rev. bras. pesqui. méd. biol
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Revista brasileira de pesquisas médicas e biológicas
Assunto da revista:
BIOLOGIA
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MEDICINA
Ano de publicação:
2003
Tipo de documento:
Article
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Congress and conference
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Project document
País de afiliação:
Brasil
País de publicação:
Brasil