Staining of intestinal protozoa with heidenhain's iron Hematoxylin
Rev. Inst. Med. Trop. Säo Paulo
; 45(1): 43-44, Jan.- Feb. 2003.
Article
em En
| LILACS
| ID: lil-330512
Biblioteca responsável:
BR1.1
ABSTRACT
Due to its unique properties, iron hematoxylin has been traditionally recommended for staining intestinal protozoa. This process can be simplified by reducing the number of steps and periods of permanence of the slides in some of the liquids used, without detriment to the quality of the results. Thus iron hematoxylin becomes adequate for routine use. Hematoxylin is a natural dye extracted from Haematoxylon campechianum, of the family Leguminosae. It must first be 'ripened', i.e. oxidized to hematein, which reacts with ferric ammonium sulphate to produce the ferric lake (iron hematoxylin), a basic dye. Iron hematoxylin most frequently stains regressively, i.e. the slides are first overstained and then differentiated
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Coloração e Rotulagem
/
Corantes
/
Eucariotos
/
Hematoxilina
Limite:
Animals
Idioma:
En
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Assunto da revista:
MEDICINA TROPICAL
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil