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Influencia del amaranto en la producción de alpha-amilasa empleando Aspergillus niger NRRL 3112 / Influence of amaranth on the production of alpha-amylase using Aspergillus niger NRRL 3112
Mariani, D. D; Lorda, G; Balatti, A. P.
Afiliação
  • Mariani, D. D; Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química. AR
  • Lorda, G; Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química. AR
  • Balatti, A. P; Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Departamento de Química. AR
Rev. argent. microbiol ; 32(4): 185-189, oct.-dec. 2000.
Artigo em Espanhol | LILACS | ID: lil-332515
Biblioteca responsável: BR1.1
ABSTRACT
In this paper the influence of the amaranth seed meal and the aeration conditions on the alpha-amylase production by Aspergillus niger NRRL 3112 were studied. The assays of selection of culture medium were carried out in a rotary shaker at 250 rpm and 2.5 cm stroke. The aeration conditions were studied in a mechanically stirred fermentor New Brunswick type. A concentration of alpha-amylase of 2750 U.Dun/ml was achieved at 120 h with a dry weight of 8.0 g/l, using a base medium with 5.0 g/l Amaranthus cruentus seed meal. In the experiment performed in a New Brunswick fermentor, the highest value was 2806 U.Dun/ml. This result was obtained after 120 h, operating at 300 rpm and an airflow of 1 l/l. min. in a limited dissolved oxygen concentration. It was determined that the increase in the agitation rate was not favorable to the enzyme production, despite that an increase was verified in the dissolved oxygen. The morphology of the microorganism, in long and ramified hyphae, was the critical factor to obtain higher levels of alpha-amylase.
Assuntos
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Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Aspergillus niger / Proteínas Fúngicas / Meios de Cultura / Corantes / Alfa-Amilases / Corante Amaranto / Indicadores e Reagentes Idioma: Espanhol Revista: Rev. argent. microbiol Assunto da revista: Microbiologia Ano de publicação: 2000 Tipo de documento: Artigo País de afiliação: Argentina Instituição/País de afiliação: Universidad Nacional de La Pampa/AR

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Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Aspergillus niger / Proteínas Fúngicas / Meios de Cultura / Corantes / Alfa-Amilases / Corante Amaranto / Indicadores e Reagentes Idioma: Espanhol Revista: Rev. argent. microbiol Assunto da revista: Microbiologia Ano de publicação: 2000 Tipo de documento: Artigo País de afiliação: Argentina Instituição/País de afiliação: Universidad Nacional de La Pampa/AR
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