Onset of in vitro rhizogenesis response and peroxidase activity in Zingiber officinale (Zingiberaceae)
Rev. biol. trop
; 49(3/4): 965-971, Sep.-Dec. 2001.
Artigo
em Inglês
| LILACS
| ID: lil-333090
Biblioteca responsável:
BR1.1
RESUMO
The induction of rooting in microshoots of Zingiber officinale cvs. Suprava, Turia local, Suruchi and V3S18 was achieved on half-strength basal Murashige and Skoog's medium supplemented with 0.5-1.0 mg/l either indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) and 2 (w/v) sucrose within 7-9 days of culture. Rooting was inhibited when the microshoots were cultured under higher concentration of auxins. The microshoots cultured on medium supplemented with NAA induced large number of thin root hairs with friable calluses within 6-7 days. Peroxidase activity was determined during root induction (0-day to the 10th day at every 2 day interval) from microshoots derived in vitro. The activity was minimum in the inductive phase (primary) and at the maximum level during the root initiative phase. These finding may be useful in monitoring the rooting behaviour in microshoots derived from different subculture and peroxidase activity as a marker for root initiation.
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Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Peroxidase
/
Brotos de Planta
/
Raízes de Plantas
/
Gengibre
/
Ácidos Indolacéticos
Idioma:
Inglês
Revista:
Rev. biol. trop
Assunto da revista:
Biologia
/
Medicina Tropical
Ano de publicação:
2001
Tipo de documento:
Artigo
País de afiliação:
Índia
Instituição/País de afiliação:
Plant Tissue Culture Laboratory/IN