Isolation and biochemical characterization of the plasma membrane of Tritrichomonas foetus
Biocell
; 20(1): 21-31, Apr. 1996.
Artigo
em Inglês
| LILACS
| ID: lil-336007
Biblioteca responsável:
BR1.1
ABSTRACT
A fraction containing plasma membrane-enriched vesicles has been prepared from Tritrichomonas foetus. Cells were ruptured using a Potter type homogenizer, under well controlled conditions, and membranes were isolated by differential centrifugation and in discontinuous sucrose gradient. This fraction was enriched 8 and 10-fold in the plasma membrane marker enzymes 5'-nucleotidase and (Na+ + K+)-dependent, ouabain-sensitive ATPase, respectively. Determination of Glucose-6-phosphatase and NADPH cytochrome c reductase activities in this fraction, indicates a minimal contamination with endoplasmic reticulum membranes. Analysis by Sodium Dodecyl Sulfate-Polyacrylamide (SDS-PAGE) gradient gel showed that the plasma membrane fraction contains several proteins with major bands corresponding to apparent molecular weights of 48, 45, 39, 37, 32, 30, 27, 23, 20, 19, 17, and 15 kDa.
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Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Membrana Celular
/
Tritrichomonas foetus
Limite:
Animais
Idioma:
Inglês
Revista:
Biocell
Assunto da revista:
Clulas
Ano de publicação:
1996
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal do Rio de Janeiro/BR