Tubulin aggregates induced by Ni2+ present microtubular characteristics
Biocell
; 19(3): 183-188, Dec. 1995.
Artigo
em Inglês
| LILACS
| ID: lil-336009
Biblioteca responsável:
BR1.1
RESUMO
Rat brain tubulin in a proper buffered solution became insoluble in the presence of 10 mM NiCl2, and sedimented at centrifugal forces as low as 500 x g for 30 min. Both nickel-sedimented and microtubular tubulin conserved 65 of colchicine binding activity after 25 days of storage at -20 degrees C. However in brain cytosol, only 9 of the initial binding activity was conserved. The electrophoretic mobility of tubulin recovered from aggregates also remained unaltered. Therefore the aggregates formed with Ni2+ share important physicochemical properties with microtubules.
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Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Tubulina (Proteína)
/
Microtúbulos
/
Níquel
Limite:
Animais
Idioma:
Inglês
Revista:
Biocell
Assunto da revista:
Clulas
Ano de publicação:
1995
Tipo de documento:
Artigo
País de afiliação:
Argentina
Instituição/País de afiliação:
Universidad Nacional de Cuyo/AR