The use of polysiloxane/polyvinyl alcohol beads as solid phase in IgG anti-Toxocara canis detection using a recombinant antigen
Mem. Inst. Oswaldo Cruz
; 98(3): 391-393, Apr. 2003. graf
Artigo
em Inglês
| LILACS
| ID: lil-340121
Biblioteca responsável:
BR1.1
RESUMO
Immunodetection of human IgG anti-Toxocara canis was developed based on ELISA and on the use of polysiloxane/polyvinyl alcohol (POS/PVA) beads. A recombinant antigen was covalently immobilized, via glutaraldehyde, onto this hybrid inorganic-organic composite, which was prepared by the sol-gel technique. Using only 31.2 ng antigen per bead, a peroxidase conjugate dilution of 1:10,000 and a serum dilution of 1:200 were adequate for the establishment of the procedure. This procedure is comparable to that which utilizes the adsorption of the antigen to conventional PVC plates. However, the difference between positive and negative sera mean absorbances was larger for this new glass based assay. In addition to the performance of the POS/PVA bead as a matrix for immunodetection, its easy synthesis and low cost are additional advantages for commercial application
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Helmintíase
/
Doenças Negligenciadas
Base de dados:
LILACS
Assunto principal:
Álcool de Polivinil
/
Ensaio de Imunoadsorção Enzimática
/
Anticorpos Anti-Helmínticos
/
Toxocara canis
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2003
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
Japão
Instituição/País de afiliação:
Asahikawa Medical Colege/JP
/
Universidade Federal de Pernambuco/BR