Catalytical properties of N-glycosylated Gluconacetobacter diazotrophicus levansucrase produced in yeast
Electron. j. biotechnol
; 7(2): 115-123, Aug. 2004. ilus, tab, graf
Article
em En
| LILACS
| ID: lil-387552
Biblioteca responsável:
CL1.1
ABSTRACT
The influence of N-glycosylation on the kinetic and catalytical properties of a bacterial fructosyltransferase (LsdA) produced in Pichia pastoris was studied. The glycosylated enzyme behaved similarly to non-glycosylated LsdA when substrate specificity, fructo-oligosaccharide (FOS) production, sucrose hydrolysis or levan formation reactions were carried out under different experimental conditions. The kinetic parameters for native or yeast-expressed LsdA determined at 60ºC, condition for the highest hydrolytic activity, followed a conventional Michaelis-Menten kinetics. Synthase activity of this levansucrase increased in water-restricted environments by addition of salt or organic solvent to the reaction mixtures.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Oligossacarídeos
/
Pichia
/
Gluconacetobacter
/
Frutose
/
Hexosiltransferases
Idioma:
En
Revista:
Electron. j. biotechnol
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2004
Tipo de documento:
Article
/
Project document
País de afiliação:
Cuba
País de publicação:
Chile