Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs
Mem. Inst. Oswaldo Cruz
; 99(6): 617-620, Oct. 2004. ilus
Artigo
em Inglês
| LILACS
| ID: lil-387911
Biblioteca responsável:
BR1.1
RESUMO
Pre-mRNA maturation in trypanosomatids occurs through a process called trans-splicing which involves excision of introns and union of exons in two independent transcripts. For the first time, we present the standardization of Trypanosoma cruzi permeable cells (Y strain) as a model for trans-splicing study of mRNAs in trypanosomes, following by RNase protection reaction, which localizes the SL exon and intron. This trans-splicing reaction in vitro was also used to analyze the influence of NFOH-121, a nitrofurazone-derivative, on this mechanism. The results suggested that the prodrug affects the RNA processing in these parasites, but the trans-splicing reaction still occurred.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Tripanossomicidas
/
Trypanosoma cruzi
/
RNA Mensageiro
/
Splicing de RNA
/
RNA de Protozoário
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2004
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Estadual Paulista/BR