Heterotopic transplantation of glycerin-preserved trachea: effect of respiratory epithelium desquamation on acute rejection
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;38(7)July 2005. ilus
Article
em En
| LILACS
| ID: lil-403862
Biblioteca responsável:
BR1.1
ABSTRACT
An effective preservation method and decreased rejection are essential for tracheal transplantation in the reconstruction of large airway defects. Our objective in the present study was to evaluate the antigenic properties of glycerin-preserved tracheal segments. Sixty-one tracheal segments (2.4 to 3.1 cm) were divided into three groups autograft (N = 21), fresh allograft (N = 18) and glycerin-preserved allograft (N = 22). Two segments from different groups were implanted into the greater omentum of dogs (N = 31). After 28 days, the segments were harvested and analyzed for mononuclear infiltration score and for the presence of respiratory epithelium. The fresh allograft group presented the highest score for mononuclear infiltration (1.78 ± 0.43, P <= 0.001) when compared to the autograft and glycerin-preserved allograft groups. In contrast to the regenerated epithelium observed in autograft segments, all fresh allografts and glycerin-preserved allografts had desquamation of the respiratory mucosa. The low antigenicity observed in glycerin segments was probably the result of denudation of the respiratory epithelium and perhaps due to the decrease of major histocompatibility complex class II antigens.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Traqueia
/
Transplante Heterotópico
/
Mucosa Respiratória
/
Crioprotetores
/
Glicerol
/
Rejeição de Enxerto
Limite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil