Genetic diversity of â-glucuronidase activity among 14 strains of the dominant human gut anaerobe Ruminococcus gnavus
Genet. mol. biol
; 29(2): 363-366, 2006. tab, graf
Article
em En
| LILACS
| ID: lil-432710
Biblioteca responsável:
BR26.1
RESUMO
Bacterial beta-glucuronidase activity in the gut increases the enterohepatic circulation of toxic compounds and plays a major role in the etiology of colon cancer. Previously, we had found that the gus gene, which codes for beta-glucuronidase in a dominant anaerobic species of the gut microbiota, Ruminococcus gnavus strain E1, is transcribed as part of an operon that includes three ORFs that code for beta-glucoside permeases of the phosphotransferase systems. This genetic organization had never been described. We have now compared beta-glucuronidase activity and the genetic environment of the gus gene in 14 strains of Ruminococcus gnavus. We found that five out of the seven glucuronidase-positive R. gnavus strains possessed another glucuronidase gene different from the gusA operon of R. gnavus E1. This dominant commensal intestinal species appears to have a high degree of genetic diversity in the genes that control beta-glucuronidase activity.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Variação Genética
/
Ruminococcus
/
Glucuronidase
/
Intestinos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Genet. mol. biol
Assunto da revista:
GENETICA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Brasil
/
França
País de publicação:
Brasil