Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs
Mem. Inst. Oswaldo Cruz
; 102(1): 97-106, Feb. 2007. tab, ilus
Article
em En
| LILACS
| ID: lil-440625
Biblioteca responsável:
BR1.1
ABSTRACT
Small nuclear RNAs (snRNAs) are important factors in the functioning of eukaryotic cells that form several small complexes with proteins; these ribonucleoprotein particles (U snRNPs) have an essential role in the pre-mRNA processing, particularly in splicing, catalyzed by spliceosomes, large RNA-protein complexes composed of various snRNPs. Even though they are well defined in mammals, snRNPs are still not totally characterized in certain trypanosomatids as Trypanosoma cruzi. For this reason we subjected snRNAs (U2, U4, U5, and U6) from T. cruzi epimastigotes to molecular characterization by polymerase chain reaction (PCR) and reverse transcription-PCR. These amplified sequences were cloned, sequenced, and compared with those other of trypanosomatids. Among these snRNAs, U5 was less conserved and U6 the most conserved. Their respective secondary structures were predicted and compared with known T. brucei structures. In addition, the copy number of each snRNA in the T. cruzi genome was characterized by Southern blotting.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Trypanosoma cruzi
/
RNA Nuclear Pequeno
/
Genoma de Protozoário
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article
/
Project document
País de afiliação:
Brasil