Biodistribution of 99m technetium- labeled creatinine in healthy rats
Braz. j. med. biol. res
; 40(6): 807-812, June 2007. graf
Artigo
em Inglês
| LILACS
| ID: lil-452678
Biblioteca responsável:
BR1.1
ABSTRACT
The distribution of creatinine, one of the toxic guanidine compounds, in various tissues has not been studied in detail by using radiolabeled creatinine. Our objective was to investigate the biodistribution of creatinine labeled with 99m technetium (99mTc) by the stannous (II) chloride method in healthy male Wistar rats. Quality controls were carried out by radio thin layer chromatography, high-performance liquid chromatography, and paper electrophoresis. The labeling yield was 85 ± 2 percent under optimum conditions (pH 7 and 100 æg stannous chloride). Rats (N = 12) were injected intravenously with 99mTc-creatinine and their blood and visceral organs were evaluated for 99mTc-creatinine uptake as percent of the injected dose per gram wet weight of each tissue ( percentID/g). The lowest amount of uptake was detected in the brain and testis. When the rate of uptake was evaluated, only the kidney showed increasing rates of uptake of 99mTc-creatinine throughout the study. Kidneys showed the highest amount of uptake throughout the study (P < 0.001 compared to all other organs), followed by liver, spleen and lung tissue.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Tecnécio
/
Compostos Radiofarmacêuticos
/
Creatinina
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2007
Tipo de documento:
Artigo
País de afiliação:
Turquia
Instituição/País de afiliação:
Dokuz Eylul University/TR
/
Ege University/TR