Discrimination of Shark species by simple PCR of 5S rDNA repeats
Genet. mol. biol
; 31(1,suppl): 361-365, 2008. ilus
Artigo
em Inglês
| LILACS
| ID: lil-484611
Biblioteca responsável:
BR26.1
ABSTRACT
Sharks are suffering from intensive exploitation by worldwide fisheries leading to a severe decline in several populations in the last decades. The lack of biological data on a species-specific basis, associated with a k-strategist life history make it difficult to correctly manage and conserve these animals. The aim of the present study was to develop a DNA-based procedure to discriminate shark species by means of a rapid, low cost and easily applicable PCR analysis based on 5S rDNA repeat units amplification, in order to contribute conservation management of these animals. The generated agarose electrophoresis band patterns allowed to unequivocally distinguish eight shark species. The data showed for the first time that a simple PCR is able to discriminate elasmobranch species. The described 5S rDNA PCR approach generated species-specific genetic markers that should find broad application in fishery management and trade of sharks and their subproducts.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Tubarões
/
Reação em Cadeia da Polimerase
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Genet. mol. biol
Assunto da revista:
Genética
Ano de publicação:
2008
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
Venezuela
Instituição/País de afiliação:
Universidad de Oriente/VE
/
Universidade Estadual Paulista/BR