Small-molecule aggregation inhibitors reduce excess amyloid in a trisomy 16 mouse cortical cell line
Biol. Res
; 41(2): 129-136, 2008. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-495746
Biblioteca responsável:
BR1.1
ABSTRACT
We have previously characterized a number of small molecule organic compounds that prevent the aggregation of the β-amyloid peptide and its neurotoxicity in hippocampal neuronal cultures. We have now evaluated the effects of such compounds on amyloid precursor protein (APP) accumulation in the CTb immortalized cell line derived from the cerebral cortex of a trisomy 16 mouse, an animal model of Down's syndrome. Compared to a non-trisomic cortical cell line (CNh), CTb cells overexpress APP and exhibit slightly elevated resting intracellular Ca2+ levéis ([Ca2+]¡). Here, we show that the compounds 2,4-dinitrophenol, 3-nitrophenol and 4-anisidine decreased intracellular accumulation of APP in CTb cells. Those compounds were non-toxic to the cells, and slightly increased the basal [Ca2+]¡. Results indícate that the compounds tested can be leads for the development of drugs to decrease intracellular vesicular accumulation of APP in trisomic cells.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Precursor de Proteína beta-Amiloide
/
Síndrome de Down
/
Doença de Alzheimer
/
Compostos de Anilina
/
Nitrofenóis
Limite:
Animais
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2008
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
/
Chile
Instituição/País de afiliação:
Universidad de Valparaíso/CL
/
Universidade Federal do Rio de Janeiro/BR
/
University of Chile/CL