A model system to study antimicrobial strategies in endodontic biofilms
J. appl. oral sci
; 17(2): 87-91, Mar.-Apr. 2009. ilus
Artigo
em Inglês
| LILACS
| ID: lil-503984
Biblioteca responsável:
BR1.1
ABSTRACT
The purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and inoculated with sufficient volume to fill the root canal during 60 days. This procedure was repeated every 72 h, always using 24-h pure culture prepared and adjusted to No. 1 MacFarland turbidity standard. Biofilm formation was analyzed by scanning electron microscopy (SEM). E. faecalis consistently adhered to collagen structure, colonized dentin surface, progressed towards the dentinal tubules and formed a biofilm. The proposed biofilm model seems to be viable for studies on antimicrobial strategies, and allows for a satisfactory colonization time of selected bacterial species with virulence and adherence properties.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Enterococcus faecalis
/
Biofilmes
/
Cavidade Pulpar
/
Modelos Biológicos
Limite:
Humanos
Idioma:
Inglês
Revista:
J. appl. oral sci
Assunto da revista:
Odontologia
Ano de publicação:
2009
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Federal University of Goiás/BR
/
Federal University of Paraná/BR
/
Pontifical Catholic University of Rio Grande do Sul/BR