Finding protein-protein interaction patterns by contact map matching
Genet. mol. res. (Online)
; 6(4): 946-963, 2007. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-520054
Biblioteca responsável:
BR26.1
ABSTRACT
We propose a novel method for defining patterns of contacts present in protein-protein complexes. A new use of the traditional contact maps (more frequently used for representation of the intra-chain contacts) is presented for analysis of inter-chain contacts. Using an algorithm based on image processing techniques, we can compare protein-protein interaction maps and also obtain a dissimilarity score between them. The same algorithm used to compare the maps can align the contacts of all the complexes and be helpful in the determination of a pattern of conserved interactions at the interfaces. We present an example for the application of this method by analyzing the pattern of interaction of bovine pancreatic trypsin inhibitors and trypsins, chymotrypsins, a thrombin, a matriptase, and a kallikrein - all classified as serine proteases. We found 20 contacts conserved in trypsins and chymotrypsins and 3 specific ones are present in all the serine protease complexes studied. The method was able to identify important contacts for the protein family studied and the results are in agreement with the literature.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Bovinos
/
Serina Endopeptidases
/
Sequência de Aminoácidos
/
Mapeamento de Interação de Proteínas
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Genet. mol. res. (Online)
Assunto da revista:
Biologia Molecular
/
Genética
Ano de publicação:
2007
Tipo de documento:
Artigo
/
Congresso e conferência
País de afiliação:
Brasil
Instituição/País de afiliação:
Embrapa Informação Tecnólogica/BR
/
Universidade Federal de Minas Gerais/BR