Mutants with heteroresistance to amphotericin B and fluconazole in Candida
Braz. j. microbiol
; 40(4): 943-951, Oct.-Dec. 2009. tab
Artigo
em Inglês
| LILACS
| ID: lil-528179
Biblioteca responsável:
BR32.1
ABSTRACT
Several studies have reported the occurrence of infections caused by Candida yeasts as well as the increasing prevalence of non albicans species. The aim of the present work is focused on the obtaining of heteroresistance to amphotericin B and fluconazole in Candida species using two distinct methodologies selection and induction. Resistant samples were obtained by selective pressure using a medium with fluconazole for growth, followed by growth in a medium with amphotericin B. The selective pressure was also created beginning with growth in amphotericin B medium followed by growth in fluconazole medium. Concomitantly, samples were submitted to the induction of resistance through cultivation in increasing concentrations of fluconazole, followed by cultivation in increasing concentrations of amphotericin B. Subsequently, the induction began with amphotericin B followed by fluconazole. Three samples resistant to fluconazole and amphotericin B were obtained, two by induction (C. glabrata and C. tropicalis) and one by selection (C. tropicalis). Both C. tropicalis originated from the same wild sample. After successive transfers for drug free medium, only the sample obtained by selection was able to maintain the resistance phenotype. These results suggest that the phenotype of heteroresitance to fluconazole and amphotericin B can be produced by two methodologies selection and induction.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Leveduras
/
Técnicas In Vitro
/
Candida
/
Candidíase
/
Resistência Microbiana a Medicamentos
/
Fluconazol
/
Farmacorresistência Fúngica Múltipla
/
Antifúngicos
Tipo de estudo:
Estudo de prevalência
/
Fatores de risco
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2009
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Instituto Adolfo Lutz/BR
/
Universidade Federal de Alfenas/BR
/
Universidade do Vale do Paraíba/BR