Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates
Braz. j. microbiol
; 41(2): 278-282, Apr.-June 2010. tab, ilus
Artigo
em Inglês
| LILACS
| ID: lil-545329
Biblioteca responsável:
BR32.1
ABSTRACT
The emergence of Extended-Spectrum Beta-Lactamase (ESBL)-producing microorganisms in Brazilian hospitals is a challenge that concerns scientists, clinicians and healthcare institutions due to the serious risk they pose to confined patients. The goal of this study was the detection of ESBL production by clinical strains of Escherichia coli and Klebsiella sp. isolated from pus, urine and blood of patients at Hospital Universitário Santa Maria, Rio Grande Sul, RS, Brazil and the genotyping of the isolates based on bla SHV genes. The ESBL study was carried out using the Combined Disc Method, while Polymerase Chain Reaction (PCR) was used to study the bla SHV genes. Of the 90 tested isolates, 55 (61.1 percent) were identified as ESBL-producing by the combined disk method. The bla SHV genes were found in 67.8 percent of these microorganisms. K. pneumoniae predominated in the samples, presenting the highest frequency of positive results from the combined disk and PCR.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doenças Negligenciadas
/
Zoonoses
Base de dados:
LILACS
Assunto principal:
Pacientes
/
Pseudomonadaceae
/
Beta-Lactamases
/
Resistência Microbiana a Medicamentos
/
Sequência de Bases
/
Reação em Cadeia da Polimerase
/
Enterobacteriaceae
/
Infecções por Enterobacteriaceae
/
Ensaios Enzimáticos Clínicos
Tipo de estudo:
Estudo diagnóstico
/
Estudo de prevalência
/
Fatores de risco
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal de Santa Maria/BR
/
Universidade de Santa Cruz do Sul/BR