Enhancement of wanlongmycin production by nitrogen ion beam implantation / Mejora de la producción de wanlongmycin por implantación de haces iónicos de nitrógeno
Electron. j. biotechnol
; 13(1): 3-4, Jan. 2010. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-559586
Biblioteca responsável:
CL1.1
ABSTRACT
In an attempt to obtain an industrial strain with higher yield of wanlongmycin, the wild strain Streptomyces griseovariabilis GAAS2507 was mutated by a novel mutagen, nitrogen ion beam with energy of 20 kilo electron volts (KeV) and dose ranging from 7.80 x 10(14) to 2.86 x 10(15) ions/cm². One mutant strain WN939 was obtained. Its yield of wanlongmycin reached 271.24 µg/mL, which was 82.10 percent higher than that of the wild strain. The mutant strain WN939 was relatively stable for the production of wanlongmycin through six successive transfers of cultures and a repeat fermentation in a 30 L fermentor. In addition, the mutant strains were investigated and divided into five types by their colony phenotypes and production of wanlongmycin. Among them, three types mutant strains exhibited positive mutation, while the other two types mutant strains exhibited negative mutation.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Oomicetos
/
Streptomyces
/
Mutagênese
/
Mutagênicos
/
Mutação
Limite:
Animais
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2010
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
China
Instituição/País de afiliação:
Guangdong Academy of Agricultural Science/CN
/
South China Agricultural University/CN