Development of trinucleotide (GGC)n SSR markers in peanut (Arachis hypogaea L)
Electron. j. biotechnol
; 13(6): 5-6, Nov. 2010. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-591909
Biblioteca responsável:
CL1.1
ABSTRACT
Cultivated peanut (Arachis hypogaea L.) is an oilseed crop of economic importance. It is native to South America, and it is grown extensively in the semi-arid tropics of Asia, Africa, and Latin America. Given an extremely narrow genetic base, efforts are being made to develop simple sequence repeat (SSR) markers to provide useful genetic and genomic tools for the peanut research community. A SSR-enriched library to isolate trinucleotide (GGC)n SSRs in peanut was constructed. A total of 143 unique sequences containing (GGC)n repeats were identified. One hundred thirty eight primer pairs were successfully designed at the flanking regions of SSRs. A suitable polymerase was chosen to amplify these GC-rich sequences. Although a low level of polymorphism was observed in cultivated peanut by these new developed SSRs, a high level of transferability to wild species would be beneficial to increasing the number of SSRs in wild species.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Arachis
/
Repetições de Microssatélites
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2010
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
China
/
Estados Unidos
Instituição/País de afiliação:
Crop Genetics and Breeding Research Unit/US
/
National Peanut Research Laboratory/US
/
Shandong Peanut Research Institute/CN
/
Tuskegee University/US
/
University of Pennsylvania/US