Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects
Electron. j. biotechnol
; 14(1): 11-12, Jan. 2011. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-591929
Biblioteca responsável:
CL1.1
ABSTRACT
A method for counting Infectious pancreatic necrosis virus (IPNV) through epifluorescence microscopy was analyzed in detail. Image processing and statistic considerations are included. The particle size of viruses was compared in different experimental conditions such as the staining of the virus with SYBR-Green I or with antibodies for specific fluorescence labeling of viral proteins. The type of surface used as mounting support was assayed as well. The results indicated that the most suitable method involves the mounting of the viral-containing suspension on a membrane filter followed by the staining with a monoclonal antibody specific for a viral protein combined with a FITC (fluorescein isothiocyanate)-conjugated secondary antibody.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Infecções por Birnaviridae
/
Aquabirnavirus
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2011
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Chile
Instituição/País de afiliação:
Universidad de Valparaíso/CL