Aumento del daño en el ADN espermático en varones mayores de 40 años / Association of age with sperm DNA fragmentation
Rev. méd. Chile
; 139(3): 306-312, mar. 2011. ilus
Artigo
em Espanhol
| LILACS
| ID: lil-597618
Biblioteca responsável:
BR1.1
ABSTRACT
Background:
There is an association between aging ana an increased number ofsperms with alterations in nuclear DNA.Aim:
To study the association between age and fragmentation of sperm DNA. Material andMethods:
Sixty two volunteers provided semen for analysis. These were separated in a group aged less than forty years and a second group aged more than forty years. Sperm DNA fragmentation was studied by TÚNEL (terminal deoxynucleotidyl transferase-mediated2'-deoxyuridine 5'-triphosphate nick end-labeling) and SCD (sperm chromatin dispersión test) assays.Results:
Compared with theiryounger counterparts, patients aged more than 40years had a higher proportion ofsperms with DNA fragmentation by TÚNEL (20 ±1.3 and24 ± 1.9 percent respectively, p < 0.05) and SCD (22 ± 1.4 and26 ± 1.6 percent respectively, p < 0.05). The results ofboth assays had a correlation coefficientofO.8. No differences between groups were observed for other seminal parameters.Conclusions:
Sperm DNA fragmentation increases with age in males.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Espermatozoides
/
Cromatina
/
Marcação In Situ das Extremidades Cortadas
/
Fragmentação do DNA
/
Análise do Sêmen
Tipo de estudo:
Fatores de risco
Limite:
Adolescente
/
Adulto
/
Humanos
/
Masculino
Idioma:
Espanhol
Revista:
Rev. méd. Chile
Assunto da revista:
Medicina
Ano de publicação:
2011
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Chile
Instituição/País de afiliação:
Universidad de Chile/CL