A preferable approach to clone hLIF cDNA from the genomic DNA

Cui, Yihong; Zhu, Guangqin; Chen, Qiuju; Wang, Yunfei; Yang, Mingming; Song, Yuxuan; Wang, Jiangang; Cao, Binyun

Cui, Yihong; Zhu, Guangqin; Chen, Qiuju; Wang, Yunfei; Yang, Mingming; Song, Yuxuan; Wang, Jiangang; Cao, Binyun.

Afiliação

Cui, Yihong; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
Zhu, Guangqin; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
Chen, Qiuju; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
Wang, Yunfei; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
Yang, Mingming; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
Song, Yuxuan; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
Wang, Jiangang; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
Cao, Binyun; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN

Electron. j. biotechnol ; 14(3): 12-12, May 2011. ilus, tab

Artigo em Inglês | LILACS | ID: lil-602989

Biblioteca responsável: CL1.1

ABSTRACT

ABSTRACT

Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA directly isolated from the mucous membrane of mouth. The hLIF sequence can be acquired within a few hours by means of amplification of each exon and splicing using overlap-PCR. Thus, the new approach developed in this study is simple, time- and cost-effective, and it is not limited to particular gene expression levels of each tissue.

Assuntos

Humanos; DNA Complementar/genética; Fator Inibidor de Leucemia/genética; Mucosa Bucal; Sequência de Bases; Clonagem Molecular; Splicing de RNA/genética; Éxons/genética; Reação em Cadeia da Polimerase Via Transcriptase Reversa

CDNA cloning; HLIF; Oral mucous membrane; Overlap PCR

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: DNA Complementar / Fator Inibidor de Leucemia / Mucosa Bucal Limite: Humanos Idioma: Inglês Revista: Electron. j. biotechnol Assunto da revista: Biotecnologia Ano de publicação: 2011 Tipo de documento: Artigo / Documento de projeto País de afiliação: China Instituição/País de afiliação: Northwest Agriculture and Forestry University/CN

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