Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze
Biol. Res
; 45(2): 131-136, 2012. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-648571
Biblioteca responsável:
CL1.1
ABSTRACT
An efficient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortified with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of high frequency microshoots in 30 days. The microshoots were successfully subcultured for shoot elongation and eventually for rooting on MS medium supplemented with indole-3-butyric acid (IBA) at 0.5mg/l. The regenerated plantlets were hardened under greenhouse conditions and transferred to garden, resulting in a 90% survival rate.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Reguladores de Crescimento de Plantas
/
Regeneração
/
Brotos de Planta
/
Folhas de Planta
/
Asteraceae
/
Organogênese Vegetal
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2012
Tipo de documento:
Artigo
País de afiliação:
Índia
/
Israel
Instituição/País de afiliação:
Karnatak University/IN
/
St. Joseph's College/IN
/
The Volcani Center/IL