Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum
Electron. j. biotechnol
; 15(5): 1-1, Sept. 2012. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-657660
Biblioteca responsável:
CL1.1
ABSTRACT
Background:
Bacterial wilt caused by Ralstonia solanacearum is the most devastating disease in peanut. Planting resistant peanut cultivars is deemed as the sole economically viable means for effective control of the disease. To understand the molecular mechanism underlying resistance and facilitate breeding process, differences in gene expression between seeds of Rihua 1 (a Virginia type peanut variety resistant to bacterial wilt) inoculated with the bacterial pathogen suspension (10(9) cfu ml-1) and seeds of the same cultivar treated with water (control), were studied using the GenefishingTM technology.Results:
A total of 25 differentially expressed genes were isolated. Expression of genes encoding cyclophilin and ADP-ribosylation factor, respectively, were further studied by real time RT-PCR, and full length cDNAs of both genes were obtained by rapid amplification of cDNA ends.Conclusions:
The study provided candidate genes potentially useful for breeding peanut cultivars with both high yield and bacterial wilt resistance, although confirmation of their functions through transgenic studies is still needed.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Arachis
/
Fatores de Ribosilação do ADP
/
Ralstonia solanacearum
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2012
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
China
Instituição/País de afiliação:
Institute of Economic Plants/CN
/
Qingdao Entry -Exit Inspection and Quarantine Bureau/CN
/
Shandong Peanut Research Institute/CN