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Kazal-type serine proteinase inhibitors in the midgut of Phlebotomus papatasi
Memorias do Instituto Oswaldo Cruz; Sigle, Leah Theresa; Ramalho-Ortigao, Marcelo.
Afiliação
  • Sigle, Leah Theresa; s.af
  • Ramalho-Ortigao, Marcelo; s.af
Mem. Inst. Oswaldo Cruz ; 108(6): 671-678, set. 2013. tab, graf
Article em En | LILACS | ID: lil-685492
Biblioteca responsável: BR1.1
ABSTRACT
Sandflies (Diptera Psychodidae) are important disease vectors of parasites of the genus Leishmania, as well as bacteria and viruses. Following studies of the midgut transcriptome of Phlebotomus papatasi, the principal vector of Leishmania major, two non-classical Kazal-type serine proteinase inhibitors were identified (PpKzl1 and PpKzl2). Analyses of expression profiles indicated that PpKzl1 and PpKzl2 transcripts are both regulated by blood-feeding in the midgut of P. papatasi and are also expressed in males, larva and pupa. We expressed a recombinant PpKzl2 in a mammalian expression system (CHO-S free style cells) that was applied to in vitro studies to assess serine proteinase inhibition. Recombinant PpKzl2 inhibited α-chymotrypsin to 9.4% residual activity and also inhibited α-thrombin and trypsin to 33.5% and 63.9% residual activity, suggesting that native PpKzl2 is an active serine proteinase inhibitor and likely involved in regulating digestive enzymes in the midgut. Early stages of Leishmania are susceptible to killing by digestive proteinases in the sandfly midgut. Thus, characterising serine proteinase inhibitors may provide new targets and strategies to prevent transmission of Leishmania.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Phlebotomus / Inibidores de Serina Proteinase / Trato Gastrointestinal / Insetos Vetores Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2013 Tipo de documento: Article / Project document País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Phlebotomus / Inibidores de Serina Proteinase / Trato Gastrointestinal / Insetos Vetores Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2013 Tipo de documento: Article / Project document País de publicação: Brasil