The Cuban scorpion Rhopalurus junceus (Scorpiones, Buthidae): component variations in venom samples collected in different geographical areas
J. venom. anim. toxins incl. trop. dis
; 19: 13-13, maio 2013.
Artigo
em Inglês
| LILACS
| ID: lil-686621
Biblioteca responsável:
BR33.1
ABSTRACT
Backgound The venom of the Cuban scorpion Rhopalurus junceus is poorly study from the point of view of their components at molecular level and the functions associated. The purpose of this article was to conduct a proteomic analysis of venom components from scorpions collected in different geographical areas of the country. Results:
Venom from the blue scorpion, as it is called, was collected separately from specimens of five distinct Cuban towns (Moa, La Poa, Limonar, El Chote and Farallones) of the Nipe-Sagua-Baracoa mountain massif and fractionated by high performance liquid chromatography (HPLC); the molecular masses of each fraction were ascertained by mass spectrometry analysis. At least 153 different molecular mass components were identified among the five samples analyzed. Molecular masses varied from 466 to 19755 Da. Scorpion HPLC profiles differed among these different geographical locations and the predominant molecular masses of their components. The most evident differences are in the relative concentration of the venom components. The most abundant components presented molecular weights around 4 kDa, known to be K+-channel specific peptides, and 7 kDa, known to be Na+-channel specific peptides, but with small molecular weight differences. Approximately 30 peptides found in venom samples from the different geographical areas are identical, supporting the idea that they all probably belong to the same species, with some interpopulational variations. Differences were also found in the presence of phospholipase, found in venoms from the Poa area (molecular weights on the order of 14 to 19 kDa). The only ubiquitous enzyme identified in the venoms from all five localities studied (hyaluronidase) presented the same 45 kD molecular mass, identified by gel electrophoresis analysis.Conclusions:
The venom of these scorpions from different geographical areas seem to be simila, and are rich in peptides that have of the same molecular masses of the peptides...
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Peptídeos
/
Fosfolipases
/
Venenos de Escorpião
/
Proteômica
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
País/Região como assunto:
Caribe
/
Cuba
Idioma:
Inglês
Revista:
J. venom. anim. toxins incl. trop. dis
Assunto da revista:
Toxicologia
Ano de publicação:
2013
Tipo de documento:
Artigo
País de afiliação:
Cuba
/
México
Instituição/País de afiliação:
Ministry of Science, Technology and Environment/CU
/
University of Havana/CU
/
University of Mexico/MX