Fatty acid composition of Drosophila photoreceptor light-sensitive microvilli
Biol. Res
; 46(3): 289-294, 2013. ilus, graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-692196
Biblioteca responsável:
CL1.1
ABSTRACT
Phototransduction, the mechanism underlying the electrical response to light in photoreceptor cells, has been thoroughly investigated in Drosophila melanogaster, an essential model in signal transduction research. These cells present a highly specialized photosensitive membrane consisting of thousands of microvilli forming a prominent structure termed a rhabdomere. These microvilli encompass the phototransduction proteins, most of which are transmembrane and exclusively rhabdomeric. Rhabdomere membrane lipids play a crucial role in the activation of the transient receptor potential ionic channels (TRP and TRPL) responsible for initiating the photoresponse. Despite its importance, rhabdomere lipid composition has not been established. We developed a novel preparation enriched in rhabdomere membranes to perform a thorough characterization of the lipidomics of Drosophila rhabdomeres. Isolated eyes (500) were homogenized and subjected to a differential centrifugation protocol that generates a fraction enriched in rhabdomere membrane. Lipids extracted from this preparation were identified and quantified by gas chromatography coupled to mass spectrometry. We found an abundance of low sterol esters (C160, C180), highly abundant and diverse triglycerides, free fatty acids, a moderate variety of mono and diacyglycerols (C160, 180, C181) and abundant phospholipids (principally C182). This preparation opens a new avenue for investigating essential aspects of phototransduction.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Células Fotorreceptoras de Invertebrados
/
Proteínas de Drosophila
/
Drosophila melanogaster
/
Canais de Potencial de Receptor Transitório
/
Ácidos Graxos
/
Microvilosidades
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2013
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Chile
Instituição/País de afiliação:
University of Chile/CL