Caries-free subjects have high levels of urease and arginine deiminase activity
J. appl. oral sci
; 22(3): 235-240, May-Jun/2014. tab
Artigo
em Inglês
| LILACS, BBO - Odontologia
| ID: lil-711711
Biblioteca responsável:
BR1.1
ABSTRACT
Objectives:
This study investigated the relationship between urease and arginine deiminase system (ADS) activities and dental caries through a cross-sectional study. Material andMethods:
Urease and ADS activities were measured in saliva and plaque samples from 10 caries-free subjects and 13 caries-active. Urease activity was obtained from the ammonia produced by incubation of plaque and saliva samples in urea. ADS activity was obtained from the ammonia generated by the arginine-HCl and Tris-maleate buffer. Specific activity was defined as micromoles of ammonia per minute per milligram of protein. Shapiro-Wilk statistical test was used to analyze the distribution of the data, and Mann-Whitney test was used to determine the significance of the data.Results:
The specific urease activity in saliva and plaque was significantly higher in individuals with low DMFT scores. ADS activity in saliva (6.050 vs 1.350, p=0.0154) and plaque (8.830 vs 1.210, p=0.025) was also higher in individuals with low DMFT scores.Conclusions:
Caries-free subjects had a higher ammonia generation activity by urease and arginine deiminase system for both saliva and plaque samples than low caries-active subjects. High levels of alkali production in oral environment were related to caries-free subjects. .
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
BBO - Odontologia
/
LILACS
Assunto principal:
Saliva
/
Urease
/
Cárie Dentária
/
Placa Dentária
/
Hidrolases
Tipo de estudo:
Estudo de etiologia
/
Estudo observacional
/
Estudo de prevalência
/
Fatores de risco
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
J. appl. oral sci
Assunto da revista:
Odontologia
Ano de publicação:
2014
Tipo de documento:
Artigo
País de afiliação:
Chile
Instituição/País de afiliação:
University of Chile/CL