Your browser doesn't support javascript.
loading
Effect of mouse antisera targeting the Phlebotomus papatasi midgut chitinase PpChit1 on sandfly physiology and fitness
Robles-Murguia, Maricela; Bloedow, Nicholas; Murray, Leigh; Ramalho-Ortigão, Marcelo.
Afiliação
  • Robles-Murguia, Maricela; Kansas State University. Department of Entomology. Manhattan. US
  • Bloedow, Nicholas; Kansas State University. Department of Entomology. Manhattan. US
  • Murray, Leigh; Kansas State University. Department of Entomology. Manhattan. US
  • Ramalho-Ortigão, Marcelo; Kansas State University. Department of Entomology. Manhattan. US
Mem. Inst. Oswaldo Cruz ; 109(8): 1064-1069, 12/2014. tab
Article em En | LILACS | ID: lil-732595
Biblioteca responsável: BR1.1
ABSTRACT
In sandflies, the absence of the peritrophic matrix (PM) affects the rate of blood digestion. Also, the kinetics of PM secretion varies according to species. We previously characterised PpChit1, a midgut-specific chitinase secreted in Phlebotomus papatasi (PPIS) that is involved in the maturation of the PM and showed that antibodies against PpChit1 reduce the chitinolytic activity in the midgut of several sandfly species. Here, sandflies were fed on red blood cells reconstituted with naïve or anti-PpChit1 sera and assessed for fitness parameters that included blood digestion, oviposition onset, number of eggs laid, egg bouts, average number of eggs per bout and survival. In PPIS, anti-PpChit1 led to a one-day delay in the onset of egg laying, with flies surviving three days longer compared to the control group. Anti-PpChit1 also had a negative effect on overall ability of flies to lay eggs, as several gravid females from all three species were unable to lay any eggs despite having lived longer than control flies. Whereas the longer survival might be associated with improved haeme scavenging ability by the PM, the inability of females to lay eggs is possibly linked to changes in PM permeability affecting nutrient absorption.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Phlebotomus / Quitinases / Proteínas de Insetos / Soros Imunes / Fatores Imunológicos / Insetos Vetores Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2014 Tipo de documento: Article / Project document País de afiliação: Estados Unidos País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Phlebotomus / Quitinases / Proteínas de Insetos / Soros Imunes / Fatores Imunológicos / Insetos Vetores Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Mem. Inst. Oswaldo Cruz Assunto da revista: MEDICINA TROPICAL / PARASITOLOGIA Ano de publicação: 2014 Tipo de documento: Article / Project document País de afiliação: Estados Unidos País de publicação: Brasil