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The consensus sequence of FAMLF alternative splice variants is overexpressed in undifferentiated hematopoietic cells
Chen, W.L.; Luo, D.F.; Gao, C.; Ding, Y.; Wang, S.Y..
Afiliação
  • Chen, W.L.; Fujian Medical University. Union Clinical Medical College. CN
  • Luo, D.F.; Fujian Medical University. Union Clinical Medical College. CN
  • Gao, C.; Fujian Medical University. Union Clinical Medical College. CN
  • Ding, Y.; Fujian Medical University. Union Clinical Medical College. CN
  • Wang, S.Y.; Fujian Medical University. Union Clinical Medical College. CN
Braz. j. med. biol. res ; 48(7): 603-609, 07/2015. tab, graf
Article em En | LILACS | ID: lil-751348
Biblioteca responsável: BR1.1
ABSTRACT
The familial acute myeloid leukemia related factor gene (FAMLF) was previously identified from a familial AML subtractive cDNA library and shown to undergo alternative splicing. This study used real-time quantitative PCR to investigate the expression of the FAMLF alternative-splicing transcript consensus sequence (FAMLF-CS) in peripheral blood mononuclear cells (PBMCs) from 119 patients with de novo acute leukemia (AL) and 104 healthy controls, as well as in CD34+ cells from 12 AL patients and 10 healthy donors. A 429-bp fragment from a novel splicing variant of FAMLF was obtained, and a 363-bp consensus sequence was targeted to quantify total FAMLF expression. Kruskal-Wallis, Nemenyi, Spearman's correlation, and Mann-Whitney U-tests were used to analyze the data. FAMLF-CS expression in PBMCs from AL patients and CD34+ cells from AL patients and controls was significantly higher than in control PBMCs (P<0.0001). Moreover, FAMLF-CS expression in PBMCs from the AML group was positively correlated with red blood cell count (rs =0.317, P=0.006), hemoglobin levels (rs =0.210, P=0.049), and percentage of peripheral blood blasts (rs =0.256, P=0.027), but inversely correlated with hemoglobin levels in the control group (rs =–0.391, P<0.0001). AML patients with high CD34+ expression showed significantly higher FAMLF-CS expression than those with low CD34+ expression (P=0.041). Our results showed that FAMLF is highly expressed in both normal and malignant immature hematopoietic cells, but that expression is lower in normal mature PBMCs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Tecido Adiposo Marrom / Metabolismo Energético Tipo de estudo: Prognostic_studies / Systematic_reviews Limite: Animals / Humans Idioma: En Revista: Braz. j. med. biol. res Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2015 Tipo de documento: Article / Project document País de afiliação: China País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Tecido Adiposo Marrom / Metabolismo Energético Tipo de estudo: Prognostic_studies / Systematic_reviews Limite: Animals / Humans Idioma: En Revista: Braz. j. med. biol. res Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2015 Tipo de documento: Article / Project document País de afiliação: China País de publicação: Brasil