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Influence of hyperprolactinemia on collagen fibers in the lacrimal gland of female mice
Araujo, Ariadne Stavare Leal; Simões, Manuel de Jesus; Verna, Carina; Simões, Ricardo Santos; Soares Júnior, José Maria; Baracat, Edmund Chada; Gomes, Regina Célia Teixeira.
Afiliação
  • Araujo, Ariadne Stavare Leal; Universidade Federal de São Paulo. São Paulo. BR
  • Simões, Manuel de Jesus; Universidade Federal de São Paulo. São Paulo. BR
  • Verna, Carina; Universidade Federal de São Paulo. São Paulo. BR
  • Simões, Ricardo Santos; Universidade Federal de São Paulo. São Paulo. BR
  • Soares Júnior, José Maria; Universidade Federal de São Paulo. São Paulo. BR
  • Baracat, Edmund Chada; Universidade Federal de São Paulo. São Paulo. BR
  • Gomes, Regina Célia Teixeira; Universidade Federal de São Paulo. São Paulo. BR
Clinics ; 70(9): 632-637, Sept. 2015. tab, ilus
Artigo em Inglês | LILACS | ID: lil-759286
Biblioteca responsável: BR1.1
ABSTRACT

OBJECTIVE:

To quantify the collagen fibers in the lacrimal gland of female mice with hyperprolactinemia.

METHODS:

Forty adult female mice were randomly divided into two groups with 20 animals each nonpregnant control (CTR1, control group, 0.2 mL of saline solution) and nonpregnant experimental (HPRL1, experimental group, 200 µg/day metoclopramide). Treatments lasted for 50 consecutive days. On day 50, 10 females from each group (control and experimental) were euthanized in the proestrus phase; then, the blood was collected and the lacrimal glands were removed. Thereafter, the remaining females were placed with the mates and continued to receive treatment with saline solution or metoclopramide. On the 6th post-coital day, 10 pregnant females from the control group (CTR2) and 10 pregnant females from the experimental group (HPRL2) were euthanized, after which blood was collected and the lacrimal glands removed. The lacrimal glands were processed for morphological analyses and collagen quantification, and prolactin and sex steroid levels were measured in the blood samples. Data were statistically analyzed using an unpaired Student t test (p<0.05).

RESULTS:

Morphological analysis revealed greater structural tissue disorganization of the lacrimal glands in the metoclopramide-treated groups. The total collagen content was significantly higher in the HPRL1 group than in the CTR1 group (p<0.05), whereas the difference between the CTR2 and HPRL2 groups was not significant.

CONCLUSION:

Our data suggest an impairment in the functioning of the lacrimal gland as a consequence of increased prolactin levels and decreased serum levels of estrogen and progesterone.
Assuntos

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Hiperprolactinemia / Colágeno / Aparelho Lacrimal Limite: Animais / Gravidez Idioma: Inglês Revista: Clinics Assunto da revista: Medicina Ano de publicação: 2015 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Universidade Federal de São Paulo/BR

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Hiperprolactinemia / Colágeno / Aparelho Lacrimal Limite: Animais / Gravidez Idioma: Inglês Revista: Clinics Assunto da revista: Medicina Ano de publicação: 2015 Tipo de documento: Artigo País de afiliação: Brasil Instituição/País de afiliação: Universidade Federal de São Paulo/BR
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