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Three days of intermittent stretching after muscle disuse alters the proteins involved in force transmission in muscle fibers in weanling rats
Gianelo, M.C.S.; Polizzelo, J.C.; Chesca, D.; Mattiello-Sverzut, A.C..
Afiliação
  • Gianelo, M.C.S.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Programa de Pós-Graduação em Reabilitação e Desempenho Funcional. Ribeirão Preto. BR
  • Polizzelo, J.C.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Programa de Pós-Graduação em Reabilitação e Desempenho Funcional. Ribeirão Preto. BR
  • Chesca, D.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Programa de Pós-Graduação em Reabilitação e Desempenho Funcional. Ribeirão Preto. BR
  • Mattiello-Sverzut, A.C.; Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Programa de Pós-Graduação em Reabilitação e Desempenho Funcional. Ribeirão Preto. BR
Braz. j. med. biol. res ; 49(2): e4118, 2016. tab, graf
Article em En | LILACS | ID: lil-766982
Biblioteca responsável: BR1.1
ABSTRACT
The aim of this study was to determine the effects of intermittent passive manual stretching on various proteins involved in force transmission in skeletal muscle. Female Wistar weanling rats were randomly assigned to 5 groups 2 control groups containing 21- and 30-day-old rats that received neither immobilization nor stretching, and 3 test groups that received 1) passive stretching over 3 days, 2) immobilization for 7 days and then passive stretching over 3 days, or 3) immobilization for 7 days. Maximal plantar flexion in the right hind limb was imposed, and the stretching protocol of 10 repetitions of 30 s stretches was applied. The soleus muscles were harvested and processed for HE and picrosirius staining; immunohistochemical analysis of collagen types I, III, IV, desmin, and vimentin; and immunofluorescence labeling of dystrophin and CD68. The numbers of desmin- and vimentin-positive cells were significantly decreased compared with those in the control following immobilization, regardless of whether stretching was applied (P<0.05). In addition, the semi-quantitative analysis showed that collagen type I was increased and type IV was decreased in the immobilized animals, regardless of whether the stretching protocol was applied. In conclusion, the largest changes in response to stretching were observed in muscles that had been previously immobilized, and the stretching protocol applied here did not mitigate the immobilization-induced muscle changes. Muscle disuse adversely affected several proteins involved in the transmission of forces between the intracellular and extracellular compartments. Thus, the 3-day rehabilitation period tested here did not provide sufficient time for the muscles to recover from the disuse maladaptations in animals undergoing postnatal development.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Fibras Musculares Esqueléticas / Força Muscular / Exercícios de Alongamento Muscular / Imobilização / Proteínas Musculares Limite: Animals Idioma: En Revista: Braz. j. med. biol. res Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Fibras Musculares Esqueléticas / Força Muscular / Exercícios de Alongamento Muscular / Imobilização / Proteínas Musculares Limite: Animals Idioma: En Revista: Braz. j. med. biol. res Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil