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Interaction of dental pulp stem cells with Biodentine and MTA after exposure to different environments
Agrafioti, Anastasia; Taraslia, Vasiliki; Chrepa, Vanessa; Lymperi, Stefania; Panopoulos, Panos; Anastasiadou, Ema; Kontakiotis, Evangelos G.
Afiliação
  • Agrafioti, Anastasia; National and Kapodistrian University of Athens. School of Dentistry. Department of Endodontics. Athens. GR
  • Taraslia, Vasiliki; National and Kapodistrian University of Athens. School of Dentistry. Department of Endodontics. Athens. GR
  • Chrepa, Vanessa; National and Kapodistrian University of Athens. School of Dentistry. Department of Endodontics. Athens. GR
  • Lymperi, Stefania; National and Kapodistrian University of Athens. School of Dentistry. Department of Endodontics. Athens. GR
  • Panopoulos, Panos; National and Kapodistrian University of Athens. School of Dentistry. Department of Endodontics. Athens. GR
  • Anastasiadou, Ema; National and Kapodistrian University of Athens. School of Dentistry. Department of Endodontics. Athens. GR
  • Kontakiotis, Evangelos G; National and Kapodistrian University of Athens. School of Dentistry. Department of Endodontics. Athens. GR
J. appl. oral sci ; 24(5): 481-486, Sept.-Oct. 2016. tab, graf
Artigo em Inglês | LILACS, BBO - Odontologia | ID: lil-797986
Biblioteca responsável: BR1.1
ABSTRACT
ABSTRACT

Objective:

The aim of the present study was to evaluate and compare the cytotoxic effects of Biodentine and MTA on dental pulp stem cells (DPSCs) and to assess cell viability and adherence after material exposure to an acidic environment. Material and

Methods:

DPSCs were cultured either alone or in contact with either Biodentine; MTA set for 1 hour; or MTA set for 24 hours. After 4 and 7 days, cell viability was measured using the MTT assay. Biodentine and MTA were also prepared and packed into standardized bovine dentin disks and divided into three groups according to the storage media (n=6/group) freshly mixed materials without storage medium (Group A); materials stored in saline (Group B); materials stored in citric acid buffered at pH 5.4 (Group C). After 24 hours, DPSCs were introduced in the wells and cell adherence, viability, and cellular morphology were observed via confocal microscopy after three days of culture. Cell viability was analyzed using repeated-measures analysis of variance test with Tukey's post hoc tests (α=0.05).

Results:

Biodentine expressed significantly higher cell viability compared with all other groups after 4 days, with no differences after 7 days. Notably, cell viability was significantly greater in 24-hour set MTA compared with 1-hour set MTA and control groups after 7 days. Material exposure to an acidic environment showed an increase in cell adherence and viability in both groups.

Conclusions:

Biodentine induced a significantly accelerated cell proliferation compared with MTA. Setting of these materials in the presence of citric acid enhanced DPSC viability and adherence.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: BBO - Odontologia / LILACS Assunto principal: Óxidos / Células-Tronco / Silicatos / Compostos de Cálcio / Compostos de Alumínio / Polpa Dentária Tipo de estudo: Estudo de avaliação Limite: Animais / Humanos Idioma: Inglês Revista: J. appl. oral sci Assunto da revista: Odontologia Ano de publicação: 2016 Tipo de documento: Artigo País de afiliação: Grécia Instituição/País de afiliação: National and Kapodistrian University of Athens/GR

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: BBO - Odontologia / LILACS Assunto principal: Óxidos / Células-Tronco / Silicatos / Compostos de Cálcio / Compostos de Alumínio / Polpa Dentária Tipo de estudo: Estudo de avaliação Limite: Animais / Humanos Idioma: Inglês Revista: J. appl. oral sci Assunto da revista: Odontologia Ano de publicação: 2016 Tipo de documento: Artigo País de afiliação: Grécia Instituição/País de afiliação: National and Kapodistrian University of Athens/GR
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