Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum
Mem. Inst. Oswaldo Cruz
; 85(2): 153-62, abr.-jun. 1990. tab, ilus
Article
em En
| LILACS
| ID: lil-90848
Biblioteca responsável:
BR15.1
RESUMO
Diverse conditions for stimulating human mononuclear cells to release thymocyte costimulatory factors were tested for their contribution to the generation of supernatants high titers of these monokines. Activity titers increased with LPS concentration, reaching a plateau between 1 and 10 microng/ml. Indomethacin did not modify the monokine, but the assay for thymocyte costimulatory activity was substantially affected by inhibitory substances produced by the monocytes in the absence of indomethacin. The use of nylon wool columns to trap the cells was shown to be effective in raising cellular densities without decreasing activity titers. As result, the yield per cell could be maintained even in the absence of serum, an important step toward the goal of purifiying bioactive from crude broths
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Timo
/
Técnicas In Vitro
/
Monócitos
/
Lipopolissacarídeos
/
Monocinas
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
1990
Tipo de documento:
Article
País de publicação:
Brasil