A method for estimating nucleotide diversity from AFLP data.
Genetics
; 151(3): 1157-64, 1999 Mar.
Article
em En
| MEDLINE
| ID: mdl-10049931
ABSTRACT
A method for estimating the nucleotide diversity from AFLP data is developed by using the relationship between the number of nucleotide changes and the proportion of shared bands. The estimation equation is based on the assumption that GC-content is 0.5. Computer simulations, however, show that this method gives a reasonably accurate estimate even when GC-content deviates from 0.5, as long as the number of nucleotide changes per site (nucleotide diversity) is small. As an example, the nucleotide diversity of the wild yam, Dioscorea tokoro, was estimated. The estimated nucleotide diversity is 0.0055, which is larger than estimations from nucleotide sequence data for Adh and Pgi.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Variação Genética
/
Polimorfismo de Fragmento de Restrição
/
Reação em Cadeia da Polimerase
/
Impressões Digitais de DNA
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Revista:
Genetics
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Japão