Reference typing report for complement component C3.
Exp Clin Immunogenet
; 15(4): 264-7, 1998.
Article
em En
| MEDLINE
| ID: mdl-10072637
ABSTRACT
A comparison of five C3 variant samples has been performed by conventional high-voltage gel electrophoresis in three laboratories (Palermo, Berlin and Mainz). Local designation was shown within SD = +/-0.75 mm migration distance in the Mainz laboratory. Methodological modifications by laboratories were not accounted for (cooling temperature, relative mobilities between runs). In parallel, all reference samples were also sequenced after exon-specific amplification. As a result, two variants with identical final designations and two variants with different mobilities were shown to conform at the molecular basis exhibiting an amino acid exchange that causes the corresponding change in relative mobility as compared to normal C3F and C3S.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Complemento C3
Limite:
Humans
Idioma:
En
Revista:
Exp Clin Immunogenet
Assunto da revista:
ALERGIA E IMUNOLOGIA
/
GENETICA
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Alemanha