Simplified preparation of a specific S. enteritidis antigen for ELISA and other immunological techniques.
Zentralbl Veterinarmed B
; 46(1): 1-7, 1999 Feb.
Article
em En
| MEDLINE
| ID: mdl-10085769
ABSTRACT
This study was conducted to prepare a specific S. enteritidis antigen (FG-Antigen) for the serological detection of S. enteritidis infections in chicken flocks. This antigen (FG-Antigen) consistent mainly of the flagellar fraction Hg and partly of the fimbrial fraction SEF14 from a S. enteritidis-phage type 4 strain. The initial steps followed in the preparation of this antigen were conducted based on a previously described procedure, which involved the application of heat at 60 degrees C. The purification process (filtration and concentration) enabled the exclusion of the cross-reaction causing LPS antigens from the preparation and allowed the retention of S. enteritidis-specific antigens composed of fimbria and Hg fractions. As a result, no cross-reaction with S. typhimurium nor with S. gallinarum was exhibited by the prepared FG-antigen. To characterize and determine its specificity, the following laboratory tests were conducted indirect ELISA, immunoblotting and a SEF14 agglutination test. In these examinations, rabbit and chicken reference sera as well as chicken field sera and absorbed hyperimmune sera against Hg-carrying serovars were used.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças das Aves Domésticas
/
Salmonella enteritidis
/
Salmonelose Animal
/
Antígenos de Bactérias
Limite:
Animals
Idioma:
En
Revista:
Zentralbl Veterinarmed B
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Alemanha