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Activity-dependent regulation of alternative splicing patterns in the rat brain.
Daoud, R; Da Penha Berzaghi, M; Siedler, F; Hübener, M; Stamm, S.
Afiliação
  • Daoud R; Max-Planck Institute of Neurobiology, D-82152 Martinsried, Germany.
Eur J Neurosci ; 11(3): 788-802, 1999 Mar.
Article em En | MEDLINE | ID: mdl-10103073
ABSTRACT
Alternative splicing plays an important role in the expression of genetic information. Among the best understood alternative splicing factors are transformer and transformer-2, which regulate sexual differentiation in Drosophila. Like the Drosophila genes, the recently identified mammalian homologues are subject to alternative splicing. Using an antibody directed against the major human transformer-2 beta isoform, we show that it has a widespread expression in the rat brain. Pilocarpine-induced neuronal activity changes the alternative splicing pattern of the human transformer-2-beta gene in the brain. After neuronal stimulation, a variant bearing high similarity to a male-specific Drosophila tra-2179 isoform is switched off in the hippocampus and is detectable in the cortex. In addition, the ratio of another short RNA isoform (htra2-beta2) to htra2-beta1 is changed. Htra2-beta2 is not translated into protein, and probably helps to regulate the relative amounts of htra2-beta1 to beta3. We also observe activity-dependent changes in alternative splicing of the clathrin light chain B, c-src and NMDAR1 genes, indicating that the coordinated change of alternative splicing patterns might contribute to molecular plasticity in the brain.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Química Encefálica / Proteínas Nucleares / Proteínas de Ligação a RNA / Processamento Alternativo / Proteínas do Tecido Nervoso Tipo de estudo: Prognostic_studies Limite: Animals / Humans / Male Idioma: En Revista: Eur J Neurosci Assunto da revista: NEUROLOGIA Ano de publicação: 1999 Tipo de documento: Article País de afiliação: Alemanha
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Química Encefálica / Proteínas Nucleares / Proteínas de Ligação a RNA / Processamento Alternativo / Proteínas do Tecido Nervoso Tipo de estudo: Prognostic_studies Limite: Animals / Humans / Male Idioma: En Revista: Eur J Neurosci Assunto da revista: NEUROLOGIA Ano de publicação: 1999 Tipo de documento: Article País de afiliação: Alemanha