Expression of chicken gizzard RLC complements the cytokinesis and developmental defects of Dictyostelium RLC null cells.
J Muscle Res Cell Motil
; 20(2): 177-86, 1999 Feb.
Article
em En
| MEDLINE
| ID: mdl-10412089
ABSTRACT
Dictyostelium RLC null cells have defects in cytokinesis and development that can be rescued by expression of either the wild type Dictyostelium RLC or an RLC mutant that cannot be phosphorylated by MLCK (S13A) (Ostrow et al., 1994). The wild type and S13A mutant LCs rescued the cells equally well, despite the fact that RLC phosphorylation increases purified Dictyostelium myosin's activity 5-fold. In this report, we assess the ability of foreign RLCs to rescue the RLC null phenotype. The RLC from smooth muscle myosin, whose activity is tightly controlled by phosphorylation, rescued the null cell phenotype. The purified hybrid myosin had an activity and motility comparable to phosphorylated Dictyostelium myosin. In contrast, cells expressing skeletal muscle RLC were deficient in cytokinesis and development, despite having an activity and motility similar to that of myosin with the unposphorylatable S13A mutant RLC. Neither foreign LC was phosphorylated when expressed in Dictyostelium. These results suggest that the level of actin-activated ATPase activity and motility is not the sole determinant of proper myosin function in vivo. Other heavy chain/light chain interactions, which occur only with the native RLC and smooth muscle RLC, appear to be necessary for optimal function.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cadeias Leves de Miosina
/
Dictyostelium
/
Moela das Aves
Limite:
Animals
Idioma:
En
Revista:
J Muscle Res Cell Motil
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Estados Unidos