Regional distribution in the mouse of proteins homologous to artery-specific antigenic proteins (ASAPs).
J Surg Res
; 85(2): 217-24, 1999 Aug.
Article
em En
| MEDLINE
| ID: mdl-10423322
ABSTRACT
We have reported the sequences of four novel proteins derived from extracts of human aortic tissue and a cDNA library from human aortic adventitia. These proteins are immunoreactive with serum immunoglobulins from patients with abdominal aortic aneurysms (AAA), and they have homologies of amino acid sequence with microfibrillar proteins of connective tissue. We are reporting separately that two of these proteins are artery-specific antigenic proteins (ASAPs) in man. The present work investigates the regional distribution of these two proteins (AAAP-40 and MatCAM-1) in mouse (E-beta-b). Antibodies were raised in rabbit against polypeptides encoding novel amino acid sequences, unique to these proteins (e.g., not reported in GenBank). Immunohistochemical studies with these two specific antibodies show conspicuous immunoreactivity of collagen-associated microfibrils in the aortic adventitia of the murine abdominal and thoracic aorta. Immunoreactive peptides were not present in brain, muscle, or kidney. These findings support the hypothesis that proteins occur in the mouse that are homologous to a unique family of aortic microfibrillar proteins in man.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aorta
/
Moléculas de Adesão Celular
/
Proteínas da Matriz Extracelular
/
Proteínas Contráteis
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Surg Res
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Estados Unidos