In vivo satellite cell activation via Myf5 and MyoD in regenerating mouse skeletal muscle.
J Cell Sci
; 112 ( Pt 17): 2895-901, 1999 Sep.
Article
em En
| MEDLINE
| ID: mdl-10444384
ABSTRACT
Regeneration of adult skeletal muscle is an asynchronous process requiring the activation, proliferation and fusion of satellite cells, to form new muscle fibres. This study was designed to determine the pattern of expression in vivo of the two myogenic regulatory factors, Myf5 and MyoD during this process. Cardiotoxin was used to induce regeneration in the gastrocnemius and soleus muscles of heterozygous Myf5-nlacZ mice, and the muscles were assayed for the presence of (beta)-galactosidase (Myf5) and MyoD. Adult satellite cells identified by M-cadherin labelling, when activated, initially express either MyoD or Myf5 or both myogenic factors. Subsequently all proliferating myoblasts express MyoD and part of the population is (beta)-galactosidase (Myf5) positive. Furthermore, we demonstrate that activated satellite cells, which express either Myf5 or MyoD, do not accumulate selectively on fast or slow muscle fibres.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regeneração
/
Transativadores
/
Proteína MyoD
/
Músculo Esquelético
/
Proteínas de Ligação a DNA
/
Proteínas Musculares
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Cell Sci
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
França