Class II DNA photolyase from Arabidopsis thaliana contains FAD as a cofactor.
Eur J Biochem
; 264(1): 161-7, 1999 Aug.
Article
em En
| MEDLINE
| ID: mdl-10447684
ABSTRACT
The major UV-B photoproduct in DNA is the cyclobutane pyrimidine dimer (CPD). CPD-photolyases repair this DNA damage by a light-driven electron transfer. The chromophores of the class II CPD-photolyase from Arabidopsis thaliana, which was cloned recently [Taylor, R., Tobin, A. & Bray, C. (1996) Plant Physiol. 112, 862; Ahmad, M., Jarillo, J.A., Klimczak, L.J., Landry, L.G., Peng, T., Last, R.L. & Cashmore, A.R. (1997) Plant Cell 9, 199-207], have not been characterized so far. Here we report on the overexpression of the Arabidopsis CPD photolyase in Escherichia coli as a 6 x His-tag fusion protein, its purification and the analysis of the chromophore composition and enzymatic activity. Like class I photolyase, the Arabidopsis enzyme contains FAD but a second chromophore was not detectable. Despite the lack of a second chromophore the purified enzyme has photoreactivating activity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arabidopsis
/
Desoxirribodipirimidina Fotoliase
/
Flavina-Adenina Dinucleotídeo
Idioma:
En
Revista:
Eur J Biochem
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Alemanha