Gene organisation, sequence variation and isochore structure at the centromeric boundary of the human MHC.
J Mol Biol
; 291(4): 789-99, 1999 Aug 27.
Article
em En
| MEDLINE
| ID: mdl-10452889
ABSTRACT
We have mapped and sequenced the region immediately centromeric of the human major histocompatibility complex (MHC). A cluster of 13 genes/pseudogenes was identified in a 175 kb PAC linking the TAPASIN locus with the class II region. It includes two novel human genes (BING4 and SACM2L) and a thus far unnoticed human leucocyte antigen (HLA) class II pseudogene, termed HLA-DPA3. Analysis of the G+C content revealed an isochore boundary which, together with the previously reported telomeric boundary, defines the MHC class II region as one of the first completely sequenced isochores in the human genome. Comparison of the sequence with limited sequence from other cell lines shows that the high sequence variation found within the classical class II region extends beyond the identified isochore boundary leading us to propose the concept of an "extended MHC". By comparative analysis, we have precisely identified the mouse/human synteny breakpoint at the centromeric end of the extended MHC class II region between the genes HSET and PHF1.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Complexo Principal de Histocompatibilidade
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Mol Biol
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Reino Unido