Alterations in macromolecular composition and cell wall integrity by ciprofloxacin in Mycobacterium smegmatis.
Lett Appl Microbiol
; 29(2): 113-7, 1999 Aug.
Article
em En
| MEDLINE
| ID: mdl-10499299
ABSTRACT
The present study has been undertaken to explore the biochemical mechanism of antimycobacterial action of a potent fluoroquinolone i.e. ciprofloxacin in Mycobacterium smegmatis. Cells grown in the presence of a subinhibitory concentration (IC50) of ciprofloxacin had a significantly lower content of all the major macromolecules i.e. DNA, RNA, proteins and lipids with maximum inhibition in DNA concentration as compared to control. Significant quantitative changes were also observed in the various chemical constituents of cell wall of ciprofloxacin grown cells. A decrease in the number of binding sites for a fluorescent probe L-anilinonapthalene-8-sulphonate (ANS) in ciprofloxacin grown cells suggested structural changes on the cell surface. Significant changes were also observed in the morphology of cells grown in the presence of ciprofloxacin by scanning electron microscopy (SEM). Our results suggest that ciprofloxacin exerts its antimycobacterial activity by affecting the cell wall as well as various macromolecules, particular DNA, the vital component for cell survival and growth.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ciprofloxacina
/
Mycobacterium smegmatis
/
Anti-Infecciosos
Idioma:
En
Revista:
Lett Appl Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Índia