Phytoestrogen content of purified, open- and closed-formula laboratory animal diets.
Lab Anim Sci
; 49(5): 530-6, 1999 Oct.
Article
em En
| MEDLINE
| ID: mdl-10551455
ABSTRACT
BACKGROUND AND PURPOSE:
Phytoestrogens exert estrogenic effects on the central nervous system, induce estrus, and stimulate growth of the genital tract of female animals. Over 300 plants and plant products, including some used in laboratory animal diets, contain phytoestrogens. Therefore, the source and concentration of phytoestrogens in rodent diets were determined.METHODS:
Twelve rodent diets and six major dietary ingredients were assayed for phytoestrogens (daidzein, genistein, formononetin, biochanin A, and coumestrol), using high-performance liquid chromatography. Three rodent diets recently formulated to reduce phytoestrogen content also were assayed.RESULTS:
Formononetin, biochanin A, and coumestrol were not detected. Soybean meal was the major source of daidzein and genistein; their concentrations were directly correlated to the percentage of soybean meal in each diet.CONCLUSIONS:
High, variable concentrations of daidzein and genistein are present in some rodent diets, and dietary phytoestrogens have the potential to alter results of studies of estrogenicity. Careful attention should be given to diet phytoestrogen content, and their concentration should be reported. A standardized, open-formula diet in which estrogenic substances have been reduced to levels that do not alter results of studies that are influenced by exogenous estrogens is recommended.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Roedores
/
Estrogênios não Esteroides
/
Ração Animal
/
Animais de Laboratório
Limite:
Animals
Idioma:
En
Revista:
Lab Anim Sci
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Estados Unidos