Molecular cloning of vacuolar H(+)-pyrophosphatase and its expression during the development of pear fruit.

The cDNA of vacuolar proton-translocating inorganic pyrophosphatase was cloned from pear fruit. It contained an open reading frame of 2,301 nucleotides, coding for a polypeptide of 767 amino acids. The level of mRNA was very low in young fruit and increased with maturation, differing from the changes in the level of polypeptide.