Protective cultures inhibit growth of Listeria monocytogenes and Escherichia coli O157:H7 in cooked, sliced, vacuum- and gas-packaged meat.
Int J Food Microbiol
; 53(1): 43-52, 1999 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-10598113
ABSTRACT
Contamination of cooked meat products with Listeria monocytogenes poses a constant threat to the meat industry. The aim of this study was therefore to investigate the use of indigenous lactic acid bacteria (LAB) as protective cultures in cooked meat products. Cooked, sliced, vacuum- or gas-packaged ham and servelat sausage from nine meat factories in Norway were inoculated with 10(3) cfu/g of a mixture of three rifampicin resistant (rif-mutant) strains of L. monocytogenes and stored at 8 degrees C for four weeks. Growth of L. monocytogenes and indigenous lactic acid flora was followed throughout the storage period. LAB were isolated from samples where L. monocytogenes failed to grow. Five different strains growing well at 3 degrees C. pH 6.2, with 3% NaCl, and producing moderate amounts of acid were selected for challenge experiments with the rif-resistant strains of L. monocytogenes. a nalidixic acid/streptomycin sulphate-resistant strain of Escherichia coli O157H7 and a mixture of three rif-resistant strains of Yersinia enterocolitica O3. All five LAB strains inhibited growth of both L. monocytogenes and E. coli O157H7. No inhibition of Y. enterocolitica O3 was observed. A professional taste panel evaluated cooked, sliced, vacuum-packaged ham inoculated with each of the five test strains after storage for 21 days at 8 degrees C. All samples had acceptable sensory properties. The five LAB strains hybridised to a 23S rRNA oligonucleotide probe specific for Lactobacillus sakei. These indigenous LAB may be used as protective cultures to inhibit growth of L. monocytogenes and E. coli O157H7 in cooked meat products.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Escherichia coli O157
/
Microbiologia de Alimentos
/
Conservação de Alimentos
/
Lactobacillus
/
Listeria monocytogenes
/
Produtos da Carne
Limite:
Animals
Idioma:
En
Revista:
Int J Food Microbiol
Assunto da revista:
CIENCIAS DA NUTRICAO
/
MICROBIOLOGIA
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Samoa americana